Record for 2012.7.16   Tian-Shyang Shoung.
pS1B3C bacteria cultured overnight are being mini-plasmid-extracted
and concentration determined.
pS1B3C bacteria also are taken to storage at -80℃ by two tubes.

plan for 2012.7.17 wednesday.
                                                                                                                                   hrs
Prepare                                                                                                                      1
Digeest the pS1B3C vector                                                                                       2
Seperate the vector and insert by gel and gel extraction and [] test                         1.5
sp1_cpp_flag; sp2_cpp_flag; sp3_cpp_flag; sp1_GLP-1 digestion                          2.5
ligation of vector and insert                                                                                      o/n
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                                                                                                                                  7hrs